Liquid chromatographic–mass spectrometric methods for analyzing antibiotic and antibacterial agents in animal food products

Public health agencies in many countries rely on detection by mass spectrometry for unambiguous identification of residues of antibiotic and antibacterial agents in animal food products for human consumption. The introduction of relatively inexpensive and robust LC–MS systems has given a strong impulse to develop determinative and confirmatory methods for the above medicines in foodstuffs. This impulse has been also dictated by thermal instability and lack of volatility of many antibiotics and antibacterials that makes the GC–MS technique of difficult application. Analytical methods developed for analyzing components of the major classes of the medicines mentioned above are here reviewed. The discussion is focused on both sample treatment and final LC–MS analysis.