Title of article
Effect of follicular size on in vitro maturation, fertilization and culture of sheep embryos
Author/Authors
واني، عبدالرئوف نويسنده Department of Animal Reproduction, Faculty of Veterinary Sciences and Animal Husbandry Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India Wani, A. R , خان، محمد ظفراله نويسنده Department of Animal Reproduction, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India Khan, M. Z , صوفي، خورشيد احمد نويسنده Department of Animal Reproduction, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India Sofi, K. A , مالك، اسلوب احمد نويسنده Department of Animal Reproduction, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India Malik, A. A , لون، فاروض احمد نويسنده Department of Animal Reproduction, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India Lone, F. A , بات، فياض احمد نويسنده Department of Animal Reproduction, Faculty of Veterinary Sciences and Animal Husbandry Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India Bhat, F. A
Issue Information
فصلنامه با شماره پیاپی 45 سال 2013
Pages
9
From page
363
To page
371
Abstract
اين پژوهش به منظور بررسي اثر اندازه فوليكول (كوچك، 2> ميلي‌متر و بزرگ، 2≤ ميلي‌متر) بر بلوغ برون‌تني، لقاح و كشت رويان‌هاي گوسفند انجام شد. تخمدان‌ها (80=n) از كشتارگاه شهر سريناگر، هند جمع‌آوري و در فلاسك گرمايشي حاوي محلول سالين نرمال داراي 50 ميكروگرم سولفات جنتامايسين در هر ميلي‌ليتر انتقال داده شد. تخمك‌ها با روش سوراخ كردن جمع‌آوري و تخمك‌هاي با كيفيت خوب و عالي به دست آمده براي انجام مرحله بلوغ در TCM-199 داراي فاكتور رشد اپيدرمي (EGF) براي 24 ساعت در شرايط 5% CO2 و هواي مرطوب C5/38 قرار داده شدند. سپس بر روي تخمك‌هاي بالغ به ترتيب فرايند لقاح و كشت به مدت شش روز انجام شد. نتايج نشان داد كه ميزان بلوغ تخمك فوليكول‌هاي بزرگ (20/85%) از فوليكول‌هاي كوچك (44/52%) بيشتر بود (P=0.002). ميزان لقاح برون‌تني تخمك‌هاي فوليكول‌هاي كوچك و بزرگ اختلاف آماري معني‌داري نداشت (به ترتيب 54/79% و 54/79%). هر چند ميزان كليواژ و مورولا در تخمك فوليكول‌هاي بزرگ (به ترتيب 69/58% و 13/34%) از فوليكول‌هاي كوچك (به ترتيب 63/38% و 32/19%) بيشتر بود (P<0.05). نتيجه اين كه تخمك‌هاي فوليكول‌هاي بزرگ برتري‌هاي تكاملي بيشتري نسبت به فوليكول‌هاي كوچك دارند و بلوغ تخمك در دوران رشد فوليكولي براي تكامل بعدي رويان گوسفند ضروري است.
Abstract
A study was conducted to investigate the effect of follicular size (small, <2 mm diameter and large, ≥2 mm) on in vitro maturation, fertilization and culture of ovine oocytes. The ovaries (n=80) were collected from abattoirs in Srinagar city, India and transported in normal saline solution containing 50 μg gentamicin sulphate per ml in a thermos flask. The oocytes were collected by puncture technique and good and fair quality oocytes obtained were subjected to maturation in TCM-199 supplemented with epidermal growth factor (EGF) for 24 h under 5% CO2 and humidified atmosphere at 38.5 C. The matured oocytes were then subsequently subjected to fertilization and culturing was done for six days. The results revealed that the maturation rate of large follicle oocytes (85.20%) was higher than small follicle oocytes (52.44%, P=0.002). In vitro fertilization rates for small and large follicle oocytes were not significantly different (79.54 and 76.65%, respectively). However, cleavage and morula rates were higher in large follicle oocytes (58.69 and 34.13%) than small follicle oocytes (38.63 and 19.32%, respectively, P<0.05). In conclusion, there is higher developmental competence of oocytes from the large follicles than small follicles and the pre-maturation of oocyte occurring during follicular growth is essential for subsequent embryonic development in sheep.
Journal title
Iranian Journal of Veterinary Research (IJVR)
Serial Year
2013
Journal title
Iranian Journal of Veterinary Research (IJVR)
Record number
1518666
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