Calibration by NMR for quantitative analysis: p-Toluenesulfonic acid as a reference substance

We have demonstrated the usefulness of p-toluenesulfonic acid (TsOH) as a reference substance to calibrate a water-soluble standard by NMR for quantitative analysis. In order to make TsOH convenient for this purpose, we first of all established its molar extinction coefficient under these conditions (ɛ = 351 at 262 nm). This was done by comparing it by quantitative NMR (qNMR) with a compound of known molar extinction coefficient, guanosine monophosphate. TsOH was then used as the standard in a second qNMR experiment to measure an aqueous solution of phosphoglycolate, a metabolite of interest in our laboratory. Perhaps due to its content of water, the purity of this compound was found to be 82%, lower than the value of 94% from the manufacturer.