Development of a new diagnostic method for galactosemia by high-performance anion-exchange chromatography with pulsed amperometric detection

We developed a new non-derivatization analytical method for the determination of galactose in the diagnosis of galactosemia by high-performance anion-exchange chromatography (HPAEC)-pulsed amperometric detection (PAD). With an anion-exchange column, the analytes were separated efficiently using 3 mM NaOH containing 1 mM NaOAc, and 200 mM NaOH was added for post-column reagent. The limit of detection (S/N = 3) and limit of quantification (S/N = 10) for galactose were 25 ng/mL and 83 ng/mL, respectively. Linear dynamic range was from 4.67 mg/dL to 53.46 mg/dL (r2 = 0.9999). The mean recovery of galactose for intra-, inter-day assays were found to be of satisfactory results (98.14–101.42%).