Processing gas chromatographic data and confidence interval calculation for partition coefficients determined by the phase ratio variation method

The phase ratio variation (PRV) method is widely used for the determination of partition coefficient values (dimensionless Henryʹs law constants) by headspace gas chromatography. Traditional data processing by linear regression has several drawbacks: potential bias introduced by linearization, absence of quality indicator of the resulting value and, in case of replicate determinations, poor utilisation of the existing measurements leading to unnecessarily large confidence intervals. The paper compares existing PRV data processing methods (linear and nonlinear regression, parametric) and derives confidence intervals for the resulting partition coefficient values. The possibility of using several series of measurements to derive a single partition coefficient value with tighter and more reliable confidence intervals is presented for all three processing methods. The methods are tested on published literature data and new experimental data for 12 volatile organic compounds in water at 25 °C. The nonlinear regression based on several series of measurements appears to be the method of choice.