T issue cultur e study in Solanum xanthocarpum for efficient micr opr opagation

Tissue culture study was carried out by culturing nodal explants of Solanum xanthocarpum in MS medium supplemented with 6 different (0.5-3.0 mg/l) concentrations of 6-Benzyl amino purine and kinetin separately and two different concentrations of IBA and IAA (0.5-1.0 mg/l). Highest percentage of response (92.5), higher mean number of shoots (9.6) and highest growth (2.8 cm) were observed in MS +2.0 mg/l BAP+ 1.0mg/l 1BA.The percent response at similar concentration of IAA was 80.6 ,mean number of shoots 6.4 and mean length of shoots 2.4cm respectively .It was further noted that MS +2.0 mg/l KN + 1.0 mg/l IBA induced multiple shoots among the explants that was 72.2% mean number 5.2 and mean length 2.0 cm respectively .Multiplication of shoot was found in the same medium having similar concentration.60-70 days plants were used for rooting in half strength MS basal medium supplemented with (0.5 5.0mg/l ) concentration of IBA . 1/2 MS + 3.5 mg/l IBA gave better response for rooting in which the percent response was 78% and the mean number of roots 12.4.Such rooted plantlets were use for hardening in the poly chamber and then transferred in poly bag containing sterile soil, Sand and compost in the ratio of 1:1:1.The survival rate was above 60%.