Simultaneous increase of mycelial biomass and intracellular polysaccharide from Fomes fomentarius and its biological function of gastric cancer intervention

In this work, the effects of submerged culture conditions and nutritional requirements on simultaneous production of mycelial biomass and intracellular polysaccharide (IPS) from a medicinal mushroom Fomes fomentarius were studied using desirability functions. Under the optimal culture condition, the production of mycelia and IPS reached 17.19 and 2.86 g l−1, respectively in a 15l stirred tank bioreactor, which were about twice than that of the basal medium. Furthermore, the ethanol extract of mycelia (EEM) and IPS had a direct antiproliferative effect on human gastric cancer cell lines SGC-7901 and MKN-45 in a dose-dependent manner. In contrast, human normal gastric cell line GES-1 was less susceptible to EEM and IPS. These results suggest that F. fomentarius may represent a promising novel approach for gastric cancer intervention.