Preparation of the β-cyclodextrin-vitamin C (β-CD-Vc) inclusion complex under high hydrostatic pressure (HHP)

In this study, a novel high hydrostatic pressure (HHP) technique was used to prepare the β-cyclodextrin-vitamin C (β-CD-Vc) inclusion complex. The inclusion ratio was positively correlated with the pressure under 300 MPa and remained at above 50.0% when the pressure was more than 300 MPa. Fourier-transform infrared spectroscopy (FI-IR) and UV–visible spectroscopy (UV–vis) analysis showed that characteristic absorption bands and the absorption peak of Vc disappeared in the spectra of the β-CD-Vc inclusion complex. Furthermore, differential scanning calorimetry (DSC) data revealed that only one endothermic peak appeared at about 138 °C in the DSC curve of the β-CD-Vc inclusion complex. These results indicate that the HHP treatment effectively induces the formation of β-CD-Vc inclusion complex.