Title of article
New method for separation and determination of denatured caseins by hydrophobic interaction chromatography
Author/Authors
Bramanti، نويسنده , , Emilia and Ferri، نويسنده , , Fabrizio and Raspi، نويسنده , , Giorgio and Lampugnani، نويسنده , , Leonardo and Carla Spinetti، نويسنده , , Maria and Miller، نويسنده , , Keith E. and Synovec، نويسنده , , Robert E.، نويسنده ,
Issue Information
ماهنامه با شماره پیاپی سال 2001
Pages
7
From page
343
To page
349
Abstract
A new method for the separation of denatured α-, β- and κ- caseins by hydrophobic interaction chromatography (HIC) is proposed. The method is based on an easy solubilization of commercial and real samples by 4.0 M guanidine thiocyanate (GdmSCN) and elution on a TSK-Gel® Phenyl-5PW column (TosoHaas) in the presence of 8.0 M urea in the mobile phase. The procedure, applied to commercial caseins and to real, raw samples (whole milk powder and fat-free yoghurt) is not expensive, it requires common high performance liquid chromatography (HPLC) instrumentation and allows the separation of caseins also in the presence of whey proteins. Quantitative results on the analysis of α-, β- and κ-caseins in real samples are also reported.
Keywords
Protein , denaturation , Guanidine thiocyanate (GdmSCN) , urea , Hydrophobic interaction chromatography , Caseins
Journal title
Talanta
Serial Year
2001
Journal title
Talanta
Record number
1641336
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