Simultaneous determination of free-form amphetamine in ratʹs blood and brain by in-vivo microdialysis and liquid chromatography with fluorescence detection

The determination of amphetamine in ratʹs blood and brain simultaneously by micordiaysis technique and HPLC/fluorescence derivatization is described. Microdiaysis is used to sample ratʹs blood and brain fluid and the amphetamine content is evaluated by HPLC/fluorescence derivatization assay. Dansyl chloride is used as fluorescence derivatization reagent. The detection limit, linearity and precision associated with this assay were evaluated. Pharmarcokinetic parameters of amphetamine in ratʹs blood and brain are also reported.