A conformationally purified α-helical peptide library

A conformationally purified peptide library was constructed based on a unique idea combining the combinatorial library concept with de novo peptide design. This peptide library was designed to allow peptides to acquire metal-binding ability when they fold into helix–loop–helix structures. Therefore, a mixture of randomized peptides was purified by immobilized metal-affinity chromatography (IMAC) to provide a library of peptides with the homogenous conformation.