Blazeispirol D and Z, as the actual intermediates of blazeispirol A biosynthesis from the cultured mycelia of the fungus Agaricus blazei

Two novel blazeispirol derivatives, blazeispirol D and Z, as the actual intermediates of blazeispirol A biosynthesis have been isolated from the cultured mycelia of Agaricus blazei (Agaricaceae). The structures were elucidated to be (20S,22S,23R,24S)-14β, 22: 22, 25-diepoxy-des-A-ergosta-5,7,9,11-tetraene-5,23-diol and (20S,22S,23R,24S)-14β,22:22,25-diepoxy-23-hydroxy-4,5-seco-ergosta-6,8,11-triene-3,5-dione by comparison of extensive 1D and 2D NMR spectral data with that of blazeispirol A. It is discussed that the compounds are the actual intermediates of blazeispirol A biosynthesis.