Title of article
Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol
Author/Authors
Seemann، نويسنده , , Myriam and Campos، نويسنده , , Narciso and Rodriguez-Concepciَn، نويسنده , , Manuel and Ibaٌez، نويسنده , , Ester and Duvold، نويسنده , , Tore and Tritsch، نويسنده , , Denis and Boronat، نويسنده , , Albert and Rohmer، نويسنده , , Michel، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2002
Pages
3
From page
1413
To page
1415
Abstract
A crude cell-free system from an Escherichia coli strain overexpressing the cluster containing the three genes yfgA, yfgB, and gcpE converted 2-C-methyl-d-erythritol 2,4-cyclodiphosphate (1) into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol (6), which most probably represents a novel intermediate in the methylerythritol phosphate pathway for isoprenoid biosynthesis. The free diol 6 was accumulated by phosphatase treatment of the crude enzyme preparation and was identified by comparison with a synthetic reference.
Keywords
Biosynthesis , 4-cyclodiphosphate , (E)-2-methylbut-2-ene-1 , 4-diol , gcpE , Isoprenoids , LytB , 2-C-methyl-d-erythritol 4-phosphate , 2-C-methyl-d-erythritol 2
Journal title
Tetrahedron Letters
Serial Year
2002
Journal title
Tetrahedron Letters
Record number
1650024
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