Using second-order calibration method based on trilinear decomposition algorithms coupled with high performance liquid chromatography with diode array detector for determination of quinolones in honey samples

A novel strategy that combines the second-order calibration method based on the trilinear decomposition algorithms with high performance liquid chromatography with diode array detector (HPLC-DAD) was developed to mathematically separate the overlapped peaks and to quantify quinolones in honey samples. The HPLC-DAD data were obtained within a short time in isocratic mode. The developed method could be applied to determine 12 quinolones at the same time even in the presence of uncalibrated interfering components in complex background. To access the performance of the proposed strategy for the determination of quinolones in honey samples, the figures of merit were employed. The limits of quantitation for all analytes were within the range 1.2–56.7 μg kg−1. The work presented in this paper illustrated the suitability and interesting potential of combining second-order calibration method with second-order analytical instrument for multi-residue analysis in honey samples.