Immunocytochemical study of NR1, NR2A and NR2B subunits of NMDA receptor in frog retina (Rana ridibunda)

The NMDA receptors are ionotropic glutamate receptors that are involved in a variety of functions in the nervous system and in particular in the retina. They are composed of NR1 and NR2 subunits. The NMDA receptors have been fairly well studied in the retina of mammals, however, there is only limited information concerning these receptors in the retinas of lower vertebrates. The aim of the present study was to investigate immunocytochemically the NR1, NR2A and NR2B subunits of the NMDA receptors in the frog retina. Six primary antibodies were used. Three of them were directed to different splice variants of the NR1 subunit and the remaining three variants directed to NR2 subunits. All antibodies showed well expressed labeling in the frog retina. The labels had a punctate character and were located mainly in the inner and the outer plexiform layers. The results obtained indicate that the NR1, NR2A and NR2B subunits of NMDA receptor may participate in the glutamatergic neurotransmission from photoreceptors to second order retinal neurons, as well as from bipolar cells to third order retinal neurons. It has been proposed that in the frog retina, several subtypes of NMDA receptors exist each involved with different functions.