Immunohistochemical characterization of the porcine nodose ganglion

This study reports for the first time the presence of several biologically active substances in the nodose ganglion of immature female pigs. The expression and distribution pattern of the studied substances was examined using a double-labeling immunofluorescence technique. In order to visualize the entire population of the ganglionic cell bodies, PGP 9.5, the pan-neuronal marker was used. The distribution and relative proportion of immunolocalized substance P, calcitonin gene related peptide, neuronal isoform of nitric oxide synthase and galanin in perikarya were evaluated. Quantitative analysis of the nodose ganglion neurons revealed that 16.187% (±1.22) of the immunoreactive PGP 9.5 was localized in the perikarya of the left-side ganglion whereas 14.234% (±3.63) of the right-side ganglion neurons expressed substance P, respectively. Accordingly, the proportion of the perikarya with calcitonin gene related peptide ranged from 12.667% (±2.66) for the left ganglion compared with 14.875% (±2.33) for the right one. With regard to the neuronal isoform of the nitric oxide synthase expression, our study revealed a population of 18.703% (±2.50) in the left and 13.336% (±1.25) in the right ganglion, respectively. The immunoreactivity for galanin was found in a relatively small population of neurons of the left nodose ganglion, where galanin-immunoreactive perikarya constituted 1.163% (±1.26), while in the contralateral ganglion 0.865% (±0.32) of total perikarya. No nerve fibers immunopositive for the above studied substances were encountered.