Charge trap in self-assembled monolayer of cytochrome b562-green fluorescent protein chimera

The self-assembly layer consisting of fusion protein is investigated in molecular-scale for the construction of bioelectronic device. Cytochrome b562 and green fluorescent protein were used as an electron acceptor and a sensitizer in the molecular layer by mimicking the photosynthesis. Self-assembled monolayer of fusion protein was formed on Au coated glass. The formation of fusion protein layer onto the Au substrate was observed by the surface plasmon resonance measurement. The surface of fusion protein layer was observed and analyzed by the scanning tunneling microscopy observation. For embodiment of the molecular electronic device, molecular arrays of fusion protein SA layer were formed by micro contact printing. Surface charge distribution of fusion protein SA layer was measured to confirm an electrical conductivity by electrostatic force microscopy observation.