Construction of biosensor for determination of galactose with galactose oxidase immobilized on polymeric mediator contains ferrocene

A galactose biosensor was developed by immobilizing galactose oxidase (GAox) on poly (glycidyl methacrylate-co-vinylferrocene) (poly (GMA-co-VFc)) film. The enzyme was immobilized via amine group onto polymeric mediator. The biosensor was characterized by the specific activity of the immobilized galactose oxidase, the apparent Michaelis constant K M app , and the stability expressed by time and a number of the performed analysis. The optimized galactose biosensor exhibited a linear response range from 2 to 20 M and detection limit of 0.1 mM toward galactose. The response time of the biosensor was 5 s. The effects of the pH and the temperature of the immobilized galactose oxidase electrode were also studied.