Title of article
Potent cytotoxic effect of the trans10, cis12 isomer of conjugated linoleic acid on rat hepatoma dRLh-84 cells
Author/Authors
Yamasaki، نويسنده , , Masao and Chujo، نويسنده , , Hitomi and Koga، نويسنده , , Yasuko and Oishi، نويسنده , , Ayana and Rikimaru، نويسنده , , Tatsuya and Shimada، نويسنده , , Mitsuo and Sugimachi، نويسنده , , Keizo and Tachibana، نويسنده , , Hirofumi and Yamada، نويسنده , , Koji، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2002
Pages
10
From page
171
To page
180
Abstract
We evaluated the cytotoxic effect of conjugated linoleic acid (CLA) on rat hepatoma dRLh-84 cells in vitro. When cells were cultured in the presence of CLA, strong cytotoxic effect on dRLh-84 cells was recognized at 1 μM level compared to the control vehicle group, and trans10, cis12-CLA but not cis9, trans11-CLA was shown to be an active isomer for inducing this effect. Increase of the sub-G1 population and activation of caspase-3 and 9 accompanied with a time-dependent cleavage of poly(ADP–ribose) polymerase were recognized in dRLh-84 cells treated with trans10, cis12-CLA. In addition, we could see nuclear fragmentation in dRLh-84 cells treated with trans10, cis12-CLA by laser scanning confocal microscopy observation. Cytotoxic effect of trans10, cis12-CLA on normal hepatocytes was weaker than on dRLh-84 cells. These data indicate trans10, cis12-CLA has a potent cytotoxic effect on dRLh-84 cells through at least in part by an apoptotic pathway.
Keywords
Conjugated linoleic acid , apoptosis , caspase , Hepatoma
Journal title
Cancer Letters
Serial Year
2002
Journal title
Cancer Letters
Record number
1817259
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