Title of article
A novel highly reactive Fab antibody for breast cancer tissue diagnostics and staging also discriminates a subset of good prognostic triple-negative breast cancers
Author/Authors
Araْjo، نويسنده , , Thaise G. and Paiva، نويسنده , , Carlos E. and Rocha، نويسنده , , Rafael M. and Maia، نويسنده , , Yara C.P. and Sena، نويسنده , , Angela A.S. and Ueira-Vieira، نويسنده , , Carlos and Carneiro، نويسنده , , Ana Paula and Almeida، نويسنده , , Juliana F. and de Faria، نويسنده , , Paulo R. and Santos، نويسنده , , Donizeti W. and Calلbria، نويسنده , , Luanda and Alcântara، نويسنده , , Tânia M. and Soares، نويسنده , , Fernando A. and Goulart، نويسنده , , Luiz R.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2014
Pages
11
From page
275
To page
285
Abstract
The discovery of novel markers for breast cancer (BC) has been recently relied on antibody combinatorial libraries and selection through phage display. We constructed a recombinant Fab library, and after selections against BC tissues, the FabC4 clone was thoroughly investigated by immunohistochemistry in 232 patients with long-term follow-up. The FabC4 ligand was determined by mass spectrometry. The FabC4 expression was associated with younger age, lack of progesterone receptor, higher histological grades and non-luminal subtypes, and it also identified a subset of good prognostic triple-negative BCs, possibly targeting a conformational epitope of Cytokeratin-10 (CK10). This new CK10-epitope specific antibody may open new possibilities in diagnostic and therapeutic strategies.
Keywords
combinatorial library , breast cancer , phage display , Recombinant Antibody
Journal title
Cancer Letters
Serial Year
2014
Journal title
Cancer Letters
Record number
1824150
Link To Document