Title of article
A CALORIMETRIC STUDY ON THE INTERACTION OF ZINC ION WITH HUMAN GROWTH HORMONE
Author/Authors
TAZIKEH، E نويسنده Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, I. R. of Iran TAZIKEH, E , SABOURY، A. A نويسنده Institute of Biochemistry and Biophysics, University of Tehran, Tehran, I. R. of Iran SABOURY, A. A , REZAEI-BEHBEHANI، G نويسنده 3Department of Chemistry, Imam Khomeini International University, Qazvin, I. R. of Iran REZAEI-BEHBEHANI, G , MOOSAVI-MOVAHEDI، A. A نويسنده Institute of Biochemistry and Biophysics, University of Tehran, Tehran, I. R. of Iran MOOSAVI-MOVAHEDI, A. A , MONAJJEMI، M نويسنده Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, I. R. of Iran MONAJJEMI, M
Issue Information
دوفصلنامه با شماره پیاپی 0 سال 2009
Pages
11
From page
317
To page
327
Abstract
A thermodynamic study on the interaction between zinc ion (Zn2+) and human growth hormone
(hGH) was studied at two temperatures of 27?C and 37?C in aqueous solution using an isothermal titration
calorimetry. It was found that there is a set of three identical and non-interacting binding sites for Zn2+ ions.
The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 1.54 mM and ?17.6 kJ
mol-1 at 27?C and 1.93 mM and ??7.1 kJ mol-1 at 37?C, respectively. To reproduce the binding parameters of
metal ion?hormone interaction over the whole range of Zn2+ concentrations a solvation theory was applied.
The binding parameters deduced from the solvation model were attributed to the structural change of hGH
and its biological activity due to the metal ion interaction.
Journal title
Iranian Journal of Science and Technology Transaction A: Science
Serial Year
2009
Journal title
Iranian Journal of Science and Technology Transaction A: Science
Record number
1847418
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