Expression of antimicrobial peptides by bovine endothelial cells

In this work, we explore if Gram-positive bacteria as Staphylococcus aureus or Gram-negative bacteria components as LPS, can induce the expression of seven antimicrobial peptides (AP) in an immortalized bovine umbilical vein endothelial cell line (BUVEC). By qPCR we determined the constitutive expression of all the AP evaluated. The stimulation with S. aureus or LPS induced the expression of lingual antimicrobial peptide (LAP), bovine β-defensin 1 (DEFB1) and bovine neutrophil β-defensin 4 (BNBD4). This expression was regulated by the autocrine production of tumor necrosis factor-α (TNF-α), indicating that bovine endothelial cells (EC) can play a more active role during infection.