Title of article
Norcholanic acids as substrates for recombinant 3β-hydroxysteroid dehydrogenase and progesterone 5β-reductase, enzymes of the 5β-cardenolide biosynthesis
Author/Authors
H.Schebitz, H.Wilkens، نويسنده , , Pia and Nothdurft، نويسنده , , Lars and Hensel، نويسنده , , Andreas and Müller-Uri، نويسنده , , Frieder and Kreis، نويسنده , , Wolfgang، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2010
Pages
4
From page
367
To page
370
Abstract
The conversion of 23-nor-5,20(22)E-choladienic acid-3β-ol and other intermediates of the putative norcholanic acid pathway of cardenolide biosynthesis by recombinant 3β-hydroxysteroid dehydrogenase from Digitalis lanata in dehydrogenation and reduction reactions was investigated. 23-nor-4,20(22)E-choladienic acid-3-one was found to be a substrate of recombinant progesterone 5β-reductases from D. lanata and Arabidopsis thaliana. The role of various substrates in cardenolide biosynthesis is discussed.
Keywords
Cardenolide biosynthesis , enzyme catalysis , 3?-Hydroxysteroid dehydrogenase , Norcholanic acid , Progesterone 5?-reductase , short-chain dehydrogenase/reductase
Journal title
Tetrahedron Letters
Serial Year
2010
Journal title
Tetrahedron Letters
Record number
1870675
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