Biohydrogen production by Anabaena sp. PCC 7120 wild-type and mutants under different conditions: Light, nickel, propane, carbon dioxide and nitrogen

Increasing awareness of environmental problems caused by the current use of fossil fuel-based energy, has led to the search for alternatives. Hydrogen is a good alternative and the cyanobacterium Anabaena sp. PCC 7120 is naturally able to produce molecular hydrogen, photosynthetically from water and light. However, this H2 is rapidly consumed by the uptake hydrogenase. tudy evaluated the hydrogen production of Anabaena sp. PCC 7120 wild-type and mutants: hupL− (deficient in the uptake hydrogenase), hoxH− (deficient in the bidirectional hydrogenase) and hupL−/hoxH− (deficient in both hydrogenases) on several experimental conditions, such as gas atmosphere (argon and propane with or without N2 and/or CO2 addition), light intensity (54 and 152 μEm−2s−1), light regime (continuous and light/dark cycles 16 h/8 h) and nickel concentrations in the culture medium. ry assay, the hupL− and hupL−/hoxH− mutants stood out over wild-type cells and the hoxH− mutant. Nevertheless, the hupL− mutant showed the best hydrogen production except in an argon atmosphere under 16 h light/8 h dark cycles at 54 μEm−2s−1 in the light period, with 1 μM of NiCl2 supplementation in the culture medium, and under a propane atmosphere. strains, higher light intensity leads to higher hydrogen production and if there is a daily 1% of CO2 addition in the gas atmosphere, hydrogen production could increase 5.8 times, related to the great increase in heterocysts differentiation (5 times more, approximately), whereas nickel supplementation in the culture medium was not shown to increase hydrogen production. The daily incorporation of 1% of CO2 plus 1% of N2 did not affect positively hydrogen production rate.