Microbial removal from the separated liquid fraction of anaerobically digested pig manure in meso-scale integrated constructed wetlands

The aim was to investigate microbial removal from the liquid fraction of anaerobically digested pig manure in meso-scale integrated constructed wetlands (ICW’s) over a 13 month period. Four treatments were investigated: T1 (standard), T2 (effluent recycling), T3 (high nutrient loading), and T4 (high flow rate). Mean counts of yeasts and moulds and spore-forming bacteria were higher in T3 and T4 than in T1 and T2 (P < 0.05). Flow through the cells reduced mean counts of coliform, yeasts and moulds and spore-forming bacteria across all treatments (P < 0.01). Counts varied with season; coliform were highest in the Summer (P < 0.001), with yeasts and moulds highest in the Summer and Autumn (P < 0.01) and spore-formers lowest in the Autumn (P < 0.001). As Salmonella was undetectable in the influent and Escherichia coli and Enterococcus were rarely detected it is difficult to make conclusions regarding pathogen removal. Further investigations using marked strains would allow pathogen tracking within the ICW’s.