Denitrification capacity of a landfilled refuse in response to the variations of in the injected leachate

Effects of different chemical oxygen demand (COD) to nitrate concentration ratios in the injected leachate on the denitrification capacity of landfilled municipal solid waste were evaluated. Results showed that the 6-year-old refuse possessed high denitrification capacity. The nitrate reduction rate increased with the increasing COD concentration in the injected leachate. When the initial COD concentration increased to 6500 mg l−1, nitrate reduction rate could reach up to 6.85 mg NO 3 - -N l - 1 h - 1 . At the initial biodegradable COD / NO 3 - -N ratio lower than the stoichiometric ratio of heterotrophic denitrification, autotrophic bacteria was the dominant microbial communities for denitrification. With the increase of COD / NO 3 - -N ratio, the primary functional denitrifier would shift from autotrophic Thiobacillus denitrificans to heterotrophic Azoarcus tolulyticus. These results suggested that the initial biodegradable COD / NO 3 - -N ratio in the injected leachate should be adjusted to higher than 6.0 for rapid in situ denitrification of 500 mg NO 3 - -N l - 1 .