Title of article
Solution state hybridization detection using time-resolved fluorescence anisotropy of quantum dot-DNA bioconjugates
Author/Authors
Giraud، نويسنده , , Gerard and Schulze، نويسنده , , Holger and Bachmann، نويسنده , , Till T. and Campbell، نويسنده , , Colin J. and Mount، نويسنده , , Andrew R. and Ghazal، نويسنده , , Peter and Khondoker، نويسنده , , Mizanur R. and Ember، نويسنده , , Stuart W.J. and Ciani، نويسنده , , Ilenia and Tlili، نويسنده , , Chaker and Walton، نويسنده , , Anthony J. and Terry، نويسنده , , Jonathan G. and C، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2010
Pages
6
From page
309
To page
314
Abstract
In this Letter, we demonstrate the application of time-resolved fluorescence anisotropy measurements to detect solution state hybridization of streptavidin conjugate (CdSe)ZnS quantum dots (QD). The study was performed on samples containing 10 nM QD incubated with 800 nM DNA. We show that the rotational correlation time of QD–DNA constructs increases significantly upon hybridization with values of 330 ns (QD-ssDNA) and 1.3 μs (QD-dsDNA), corresponding to a diameter of 14 nm and 23 nm respectively. The present study opens a new modality for hybridization detection using quantum dots.
Journal title
Chemical Physics Letters
Serial Year
2010
Journal title
Chemical Physics Letters
Record number
1928140
Link To Document