Title of article
Effect of inulin on efficient production and regulatory biosynthesis of bacillomycin D in Bacillus subtilis fmbJ
Author/Authors
Qian، نويسنده , , Shiquan and Lu، نويسنده , , Hedong and Meng، نويسنده , , Panpan and Zhang، نويسنده , , Chong and Lv، نويسنده , , Fengxia and Bie، نويسنده , , Xiaomei and Lu، نويسنده , , Zhaoxin، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2015
Pages
8
From page
260
To page
267
Abstract
The effect of inulin on the production of bacillomycin D and the levels of mRNA of bacillomycin D synthetase genes: bmyA (BYA), bmyB (BYB), bmyC (BYC), the thioesterase gene (TE) and regulating genes: AbrB, ComA, DegU, PhrC, SigmaH and Spo0A in Bacillus subtilis fmbJ were investigated. The production of bacillomycin D was enhanced with the increase of biomass concentration. The maximum production and productivity of bacillomycin D were found to be 1227.49 mg/L and 10.23 mg/L h. Inulin significantly improved the expression of bacillomycin D synthetase genes: bmyA (BYA), bmyB (BYB), bmyC (BYC) and the thioesterase gene (TE). Also, inulin up-regulated ComA, DegU, SigmaH and Spo0A and therefore promoted the high production of bacillomycin D. Our results provided a practical approach for efficient production of bacillomycin D and a meaningful explanation for regulatory mechanism of bacillomycin D biosynthesis.
Keywords
Gene expression , Regulatory mechanism , Fed-batch fermentation , inulin , Bacillomycin D production
Journal title
Bioresource Technology
Serial Year
2015
Journal title
Bioresource Technology
Record number
1939048
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