A rapid method for separation of anomeric saccharides using a cyclodextrin bonded phase and for investigation of mutarotation

HPLC separation of anomeric sugars using a β-cyclodextrin bonded phase and an eluent ethyl acetate/methanol/water has been achieved. HPLC is a useful method for analytically separating the anomers of sugars in a mutarotated system and is a good supplement to the nuclear magnetic resonance. By means of this technique investigations of the mutarotation of D-glucose gave 36% α-form and 64% β-form. The pH dependence of mutarotation in an organic solvent was examined and discussed. Further mono- and disaccharides were separated in anomers. Furanoid forms of the sugar can also be determined by HPLC.