A novel minisequencing test for species identification of salted and dried products derived from species belonging to Gadiformes

The aim of the present study is to develop an assay for the specific identification of Gadus morhua, Gadus macrocephalus, Gadus ogac, Molva molva and Brosme brosme targeting sequences of the cytochrome b (cyt b) gene of mitochondrial DNA. The primers used in the preliminary PCR were designed in well conserved regions upstream and downstream of the diagnosis sites. They successfully amplified a conserved 188 bp region from the cyt b gene of all the species taken into consideration. The sites of diagnosis have been interrogated using a minisequencing reaction and capillary electrophoresis. All the results of the test were confirmed by fragment sequencing.