Title of article
Generation of anti-zearalenone scFv and its incorporation into surface plasmon resonance-based assay for the detection of zearalenone in sorghum
Author/Authors
Edupuganti، نويسنده , , Soujanya Ratna and Edupuganti، نويسنده , , Om Prakash and OʹKennedy، نويسنده , , Richard، نويسنده ,
Issue Information
ماهنامه با شماره پیاپی سال 2013
Pages
7
From page
668
To page
674
Abstract
Recombinant scFvs to zearalenone were isolated by phage display, expressed in Escherichia coli and the analytical sensitivity of selected scFvs determined by competitive inhibition enzyme-linked immunoassay (CI-ELISA) and surface plasmon resonance (SPR)-based inhibition assay. The results demonstrated higher sensitivity for the SPR-based assay compared to CI-ELISA. The applicability of the SPR-based assay for the detection of zearalenone in food samples was evaluated using sorghum.
Keywords
surface plasmon resonance (SPR) , sorghum , Zearalenone (ZEN) , Single chain antibody fragment (scFv) , Cross reactivity
Journal title
Food Control
Serial Year
2013
Journal title
Food Control
Record number
1948806
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