Title of article
Determination of tryptophan by high-performance liquid chromatography of alkaline hydrolysates with spectrophotometric detection
Author/Authors
Yust، نويسنده , , Mar??a M. and Pedroche، نويسنده , , Justo and Gir?n-Calle، نويسنده , , Julio and Vioque، نويسنده , , Javier and Mill?n، نويسنده , , Francisco and Alaiz، نويسنده , , Manuel، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
4
From page
317
To page
320
Abstract
A procedure for quantitation of tryptophan in feedstuff is described. It consist of hydrolysis in sodium hydroxide at 100 °C for 4 h, neutralization of the resulting hydrolysate to pH 7, dilution with sodium borate buffer (pH 9), and analysis by reverse-phase high-performance liquid chromatography with spectrophotometric determination of tryptophan at 280 nm. The recovery of tryptophan from lysozyme, added to some feedstuff before hydrolysis, ranges from 98.6 to 100%. A reduction of the time of analysis has been achieved as compared to previous methods for analysis of tryptophan.
Keywords
Tryptophan , Sodium hydrolysis , High-performance liquid chromatography , Feedstuff
Journal title
Food Chemistry
Serial Year
2004
Journal title
Food Chemistry
Record number
1950904
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