α-Amylase inhibitory effect of 3β-olean-12-en-3-yl (9Z)-hexadec-9-enoate isolated from Spondias mombin leaf

Spondias mombin is a traditional herb used in the treatment of diabetes mellitus by traditional healers in southwest Nigeria. In this study, we investigated the antidiabetic activity using α-amylase inhibitory assay and isolated an active compound. The bioactivity assay-guided study demonstrated the presence of an α-amylase inhibitory fraction from S. mombin leaf. An active compound, 3β-olean-12-en-3-yl (9Z)-hexadec-9-enoate was also studied. This is reported, from this plant, for the first time. The methanol extract, diethyl ether fraction and the isolated compound exhibited significant enzyme inhibitory activity against Aspergillus oryzae α-amylase. Our study revealed, for the first time, the isolation and α-amylase inhibitory activity of 3β-olean-12-en-3-yl (9Z)-hexadec-9-enoate from S. mombin leaf.