Gold nanorods–hemoglobin bio-conjugate: Spectroscopy studies

The interactions between nanostructures and biomolecules are intensely studied. Noble metal nanoparticles can modify the optical response of macromolecules. In this study, we analyzed by spectroscopic methods (UV–Vis Spectroscopy, Steady State Fluorescence, and FTIR) the bio-conjugation of horse hemoglobin with gold nanorods. Upon the formation of the gold nanorods–hemoglobin complex, the fluorescence signal increases, through Metal-enhanced fluorescence phenomenon.