Title of article
Interaction of tannase from Aspergillus niger with polycations applied to its primary recovery
Author/Authors
Durلn، نويسنده , , Luis V. Rodrيguez and Spelzini، نويسنده , , Darيo and Boeris، نويسنده , , Valeria and Aguilar، نويسنده , , Cristَbal N. and Picَ، نويسنده , , Guillermo A.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2013
Pages
5
From page
480
To page
484
Abstract
The interaction of tannase (TAH) with chitosan, polyethyleneimine and Eudragit®E100 was studied. It was found that TAH selectively binds to these polycations (PC), probably due to the acid nature of the target protein. TAH could interact with these PC depending on the medium conditions. The effect of the interaction on the secondary and tertiary structure of TAH was assayed through circular dichroism and fluorescence spectroscopy. TAH was recovered from Aspergillus niger culture broth by means of precipitation and adsorption using chitosan.
Keywords
Tannase , Interaction , Purification , Adsorption , polyelectrolytes , Precipitation
Journal title
Colloids and Surfaces B Biointerfaces
Serial Year
2013
Journal title
Colloids and Surfaces B Biointerfaces
Record number
1977071
Link To Document