Isolation of antioxidant phytoconstituents from the seeds of Lens culinaris Medik

Lens culinaris Medik (Leguminosae) is an annual, bushy and herbaceous plant cultivated globally for its edible seeds. A methanolic extract of the seeds contained four new antioxidant compounds, namely β-sitosteryl-3-(2′-n-eicosanyloxy)-benzoate (3), n-octadec-9-enoyl-1-β-d-glucurano-pyranoside (4) α-d-galactopyranosyl-(6→1′)-α-d-galactopyranosyl-(6′→1″)-α-d-galactopyranosyl-(6″→1‴)-α-d-galactopyranoside (5) and benzoyl-O-α-d-glucopyranosyl-(2a→1b)-O-α-d-glucopyranosyl-(2b→1c)-O-α-d-glucopyranosyl-(6c→1d)-O-α-d-glucopyranosyl-(6d→1e)-O-α-d-gluco-pyranoside (6) along with two known compounds n-heptadecanyl n-octadec-9-enoate (1) and β-sitosterol (2) on the basis of chromatographic and spectral data analytical techniques. Compound 3 showed significant antioxidant activity compared to compounds 4, 5, and 6.