Title of article
Partitioning of lactate dehydrogenase from bovine heart crude extract by polyethylene glycol–citrate aqueous two-phase systems
Author/Authors
de Araْjo، نويسنده , , Rosângela Ferreira Frade and Porto، نويسنده , , Tatiana Souza and Martins، نويسنده , , Danyelly Bruneska Gondim and Dutra، نويسنده , , Rosa Fireman and Porto، نويسنده , , Ana Lْcia Figueiredo and Filho، نويسنده , , José Luiz de Lima Filho، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2011
Pages
5
From page
46
To page
50
Abstract
Aqueous two-phase systems (ATPS) composed of polyethylene glycol (PEG)–citrate have been used for enzyme partitioning studies. The behavior of lactate dehydrogenase (LDH) from bovine heart crude extract was analyzed using a two-level factorial design in which the PEG molar mass and concentration, the citrate concentration were selected as independent variables, while the purification factor, the partition coefficient (K) and the activity yield were selected as responses. The statistical analysis revealed the effect of PEG molar mass on K. LDH exhibited a better partitioning toward PEG-rich phase and the highest K value (1079.81) was obtained with 42% (w/w) PEG 400 and 7.5% (w/w) citrate concentration. PEG molar mass also influenced the purification factor of the enzyme in the top phase. Possibly these ATPS remove inhibitors present in the extract affording higher enzyme yield.
Keywords
Partitioning , Citrate , polyethylene glycol , Lactate dehydrogenase , Aqueous two-phase system
Journal title
Fluid Phase Equilibria
Serial Year
2011
Journal title
Fluid Phase Equilibria
Record number
1988262
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