Title of article
A convenient method to generate methylated and un-methylated control DNA in methylation studies
Author/Authors
-، - نويسنده Biotechnology department, Shahid Beheshti University of Medical Sciences, Tehran, Iran Manoochehri, Mehdi , -، - نويسنده Biotechnology department, Shahid Beheshti University of Medical Sciences, Tehran, Iran Bandehpour, Mojgan , -، - نويسنده Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Kazemi, Bahram
Issue Information
فصلنامه با شماره پیاپی 0 سال 2013
Pages
5
From page
57
To page
61
Abstract
-
Abstract
Methylated and un-methylated control DNA is an important part of DNA methylation studies. Although human and mouse DNA methylation control sets are commercially available, in case of methylation studies on other species such as animals, plants, and bacteria, control sets need to be prepared. In this paper a simple method of generating methylated and un-methylated control DNA is described. Whole genome amplification and enzymatic methylation were performed to generate un-methylated and methylated DNA. The generated DNA were confirmed using methylation sensitive/dependant enzymes, and methylation specific PCR. Control reaction assays confirmed the generated methylated and un-methylated DNA.
Journal title
Molecular Biology Research Communications
Serial Year
2013
Journal title
Molecular Biology Research Communications
Record number
2029058
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