Fabrication of protein G LB film for immunoglobulin G immobilization

For the development of an immunosensor and protein chip with high efficiency, the thin film of protein G to has specific affinity to the Fc portion of immunoglobulin G (IgG) was fabricated by using the Langmuir–Blodgett (LB) technique. The formation of protein G LB film on a solid surface was confirmed by surface plasmon resonance (SPR), and the surface morphologies of the protein G LB film were observed by using atomic force microscopy (AFM). The IgG binding to protein G LB film and antigen binding to immobilized IgG were verified by SPR measurement.