Bioconjugate polymer by photo-iniferter approach: Hydrophilic random or block copolymer-coated surface

The fabrication of a high-performance polymer material is important, since it would have the potential of being utilized as a highly effective platform for bioconjugation. In the present study, hydrophilic random or block polymers were designed by a photo-iniferter approach. As typical monomers, n-butyl methacrylate (BMA) and acrylic acid (AA) were selected to prepare a surface modifier on a substrate. As surface modifiers, the monomers were copolymerized in block and random sequences, and the resulting polymer-coated surfaces were compared in terms of surface wet ability and the bioconjugation of the fluorescence-labeled protein. The most favorable characteristic of the resulting polymers was that they offered a dual function: the stability of the adsorbed polymer layer and bioconjugation via functional groups. In the present study, we focused on the physical properties of the polymer-coated surface. Bioconjugation and cell viability were also evaluated.