Title of article
Spectrophotometric detection of histidine and lysine using combined enzymatic reactions
Author/Authors
Kugimiya، نويسنده , , Akimitsu and Takamitsu، نويسنده , , Emi، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2013
Pages
4
From page
4867
To page
4870
Abstract
An amino acid-sensing system with absorption spectrophotometric detection was developed. To ensure specific recognition of each amino acid, aminoacyl-tRNA synthetases were employed and the concentration of NADH produced by way of several enzymatic reactions was measured. Using this detection system, from 1.5 to 55 μM of histidine and from 15 to 95 μM of lysine could be measured selectively in HEPES-KOH buffer (pH 8.0).
Keywords
Clinical examination , ?-Nicotinamide adenine dinucleotide , aminoacyl-tRNA synthetase , Biosensing , Amino acid , Food industry
Journal title
Materials Science and Engineering C
Serial Year
2013
Journal title
Materials Science and Engineering C
Record number
2103712
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