Title of article
A colorimetric assay for the quantification of brook trout (Salvelinus fontinalis) lymphocyte mitogenesis
Author/Authors
Daly، نويسنده , , J.G. and Moore، نويسنده , , A.R. and Olivier، نويسنده , , G.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 1995
Pages
9
From page
265
To page
273
Abstract
A colorimetric assay using the dye MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) was developed to quantify the proliferative responses of brook trout (Salvelinus fontinalis) peripheral blood and kidney lymphocytes. Mitogenic responses were assayed using the mitogens LPS, PHA, ConA and formalin-killedAeromonas salmonicida. Optimal mitogenesis occurred only in the presence of homologous plasma. The assay proved to be a simple and reproducible alternative to the traditional3H-thymidine incorporation assay.
Keywords
MTT , Lymphocytes , Mitogens , Salvelinus fontinalis , Salmonid , Aeromonas salmonicida
Journal title
Fish and Shellfish Immunology
Serial Year
1995
Journal title
Fish and Shellfish Immunology
Record number
2106089
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