Title of article
Study of a room temperature phosphorescence phenomenon to allow the detection of aflatoxigenic strains in culture media
Author/Authors
Rojas-Durلn، نويسنده , , T.R. and Fente، نويسنده , , C.A. and Vلzquez، نويسنده , , B.I. and Franco، نويسنده , , C.M. and Sanz-Medel، نويسنده , , A. and Cepeda، نويسنده , , A.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2007
Pages
10
From page
149
To page
158
Abstract
A novel screening method based on room temperature phosphorescence (RTP) for the visual detection of aflatoxigenic strains from Aspergillus genus is described. Strains were cultured on media widely used in food mycology to which methyl-β-cyclodextrin plus bile salts (0.6% sodium deoxycholate) were added. Aflatoxin production was readily detectable after 3 days of incubation at 28 °C by RTP emission from the mycelium of aflatoxigenic strains observed after exposure to UV light. The method was tested on thirty-two Aspergillus sp. strains. The phosphorescence phenomenon was reproduced in vitro by immobilizing aflatoxin B1 on ion exchange resin beads.
Keywords
Aflatoxin production , Aspergillus sp. , cyclodextrin , UV light , fluorescence , Room temperature phosphorescence , Bile salts
Journal title
International Journal of Food Microbiology
Serial Year
2007
Journal title
International Journal of Food Microbiology
Record number
2112651
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