Title of article
Evaluation of the enzymes involved in primary nitrogen metabolism in Tilia platyphyllos-Tuber borchii ectomycorrhizae
Author/Authors
Pierleoni، نويسنده , , Raffaella and Vallorani، نويسنده , , Luciana and Sacconi، نويسنده , , Cinzia and Sisti، نويسنده , , Davide and Giomaro، نويسنده , , Giovanna and Stocchi، نويسنده , , Vilberto Stocchi، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2001
Pages
4
From page
1111
To page
1114
Abstract
No information is available about Tuber borchii Vittad. ammonium metabolism during its life cycle, which involves the succession of three distinct phases. In this direction, the levels of glutamine synthetase (GS; EC 6.3.1.2), glutamate synthase (GOGAT; EC 1.4.1.13-14) and glutamate dehydrogenase (GDH; EC 1.4.1.2-4) were evaluated in Tilia platyphyllos Scop.-Tuber borchii Vittad. ectomycorrhizae, free living mycelium and non-inoculated roots. In the plant roots, GS shows high specific activity and only NADH-GDH (EC 1.4.1.2) is detectable; on the other hand, in free living mycelium GS and NADPH-GDH (EC 1.4.1.4) can be detected. Ectomycorrhizal metabolism was found to be deeply influenced by the two symbiotic partners. In fact, GS and both forms of GDH are present and their specific activities are higher than those found in the plant root and in the mycelial cells.
Keywords
primary nitrogen metabolism , Tilia platyphyllos , glutamine synthetase , ectomycorrhizae , glutamate dehydrogenase , glutamate synthase , Tuber borchii
Journal title
Plant Physiology and Biochemistry
Serial Year
2001
Journal title
Plant Physiology and Biochemistry
Record number
2120315
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