Antibacterial effects of enniatins J1 and J3 on pathogenic and lactic acid bacteria

Enniatins (ENs) are N-methylated cyclohexadepsipeptides, secondary metabolites produced by various species of the genus Fusarium. They are known to act as antifungal, antiyeast and antibacterial and to possess antiinsecticidal and phytotoxic properties. In this study we evaluated for the first time the antibiotic effect of pure fractions of EN J1 and J3 on several pathogenic strains and lactic acid bacteria. s J1 and J3 were purified from the fermentation extract of Fusarium solani growth on solid medium of wheat kamut, using the technique of the low pressure liquid chromatography (LPLC) followed by a semipreparative liquid chromatography (LC). The purity and the structure of the isolated compound were confirmed by electrospray ionization–mass spectrometry study-linear ion trap (ESI–MS-LIT). e of both chromatographic techniques have permitted to produce and purify 47 mg of the En J1 and 50 mg of the EN J3 with a mean purity of 98% completely characterized with the technique of the ESI–MS-LIT. ial bioassay analyses were carried out by incubation in MRSA and TSA for acid lactic and pathogenic bacteria, respectively during 24 h at 37 °C. None of the tested strains were inhibited by a 1 ng dose of EN J1 and J3. These compounds were only not effective against Listeria monocytogenes, Pseudomonas aeruginosa and Salmonella enteric. This study highlight ENs J1 and J3 could be potentially effective antibacterial agents against several pathogenic and lactic acid bacteria.