Cleaning From the Inside: Biodegradation of Organophosphate Pesticides by Pseudomonas plecoglossicida

Diazinon is one of the most widely used organophosphrous pesticides in the world may affect animals and human. This study was done to assess whether Pseudomonas plecoglossicida can grow on diazinon as the sole carbon metabolic source in laboratory and agricultural soil. Serial dilutions of pure and commercial diazinon in minimal salt medium were made and 2.5 micro liters of these liquid cultures before and after inoculation with Pseudomonas plecoglossicida were injected to a high performance liquid chromatography column, and subsequently the bacterial growth along with the concentration of diazinon were measured by high performance liquid chromatography (HPLC). Results of this study indicated that Pseudomonas plecoglossicida can grow not only in Minimal salt Medium but also in the diazinon contaminated soil. Our findings showed that Pseudomonas plecoglossicida grows in Minimal salt Medium supplemented with serial dilutions of diazinon. Furthermore, this bacterium is capable to biodegrade the residual diazinon in the agricultural soil.