Characterization of Specific RAPD Markers of Virulence in Tri-chomonas vaginalis Isolates
MARTINEZ-FRAGA، JORGE نويسنده , , ROJAS، L?zara نويسنده Dept. of Parasitology, Institute of Tropical Medicine Pedro Kour?, Havana, Cuba ROJAS, L?zara , SARIEGO، Idalia نويسنده Dept. of Parasitology, Institute of Tropical Medicine Pedro Kour?, Havana, Cuba SARIEGO, Idalia , FERN?NDEZ-CALIENES، Aymé نويسنده Dept. of Parasitology, Institute of Tropical Medicine Pedro Kour?, Havana, Cuba FERN?NDEZ-CALIENES, Aymé
Background: As for human trichomoniasis the host-parasite relationship is very complex, and the broad ranges of clinical symptoms are unlikely be attributable to a single pathogenic mechanism. Specific Random Amplified Polymorphic DNA (RAPD) markers of 490 bp, 720 bp and 460 bp using the primers Tv-5, OPA-6 and OPA-11, respectively, were reported. This was the first description of possible genetic virulence markers of the infection by T. vaginalis. The aim of this study was to characterize the specific RAPD markers in order to elucidate their importance on virulence of this illness.
Methods: The selected specific RAPD fragments were cloned and sequenced. The obtained sequences were compared by the BLAST algorithm.
Results: The nucleotide sequence of the Tv-5490 RAPD marker exhibited significant similarity to T. vaginalis hypothetical G3 leucine rich repeat (LRR) family protein (e-value: 6e-14) and Giardia lamblia leucine rich repeat protein 1 virus receptor protein (e-value: 6e-14 and 2e-12) ; however, the OPA-6720 and OPA-11460 showed no significant similarity with any coding published sequence. All the evaluated strains showed the presence of the LRR gene.
Conclusion: These results demonstrate a possible role of this gene in the virulence of T. vaginalis and in the parasite infection with Trichomonas virus as a possible virus receptor. Further analysis of this gene and encoded protein will allow determining the role that they play in the isolates virus susceptible or resistant phenotypes.