Immunological analysis of aerobic bioreactor bovine theileriosis vaccine.
Habibi، Gholamreza نويسنده Student of Mathematical Statistics, Faculty of Basic Sciences, Imam Khomeini International University, Qazvin, IRAN , , Esmaeil-Nia، Kasra نويسنده Department of Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Karaj, Iran. Esmaeil-Nia, Kasra , Izadi، Hasan نويسنده Department of Foot and Mouth Disease Vaccine production, Razi Vaccine and Serum Research Institute, Karaj, Iran. Izadi, Hasan , Ataie Amarloie، Orang نويسنده Department of Clinical Sciences, Faculty of Veterinary Sciences, Islamic Azad University, Karaj, Iran. Ataie Amarloie, Orang , Afshari، Asghar نويسنده Department of Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Karaj, Iran. Afshari, Asghar , Bordbar، Nadia نويسنده Department of Quality Control of Biological Products, Razi Vaccine and Serum Research Institute, Karaj, Iran. Bordbar, Nadia
Background: In this study, the pilot production of aerobic bioreactor tropical theileriosis vaccine was optimized with the aim of immunological assays for further mass production.
Methods: We have shown earlier the delayed type hypersensitivity (DTH) assay could be used for evaluating the immunity and memory cells against specific Theileria antigen in vaccinated animals. In addition, TNF-α is the principle cytokine in modulating the cytotoxic activity of cytotoxic T-lymphocytes (CTL). Immunological analysis of the vaccine was performed by using two cell mediated immunity(CMI) in vitro and in vivo DTH test (Theilerin) and TNF-α assay.
Results: The results of immune responses of susceptible immunized cattle by bioreactor vaccine in comparison with conventional flask vaccine revealed a significant stimulation of immune cells by transcription of high level of TNF-α and positive reaction against Theileria antigen in Theilerin skin test (DTH).
Conclusion: The equal immunological results achieved in both above mentioned vaccines verified the satisfactory immunity for aerobic bioreactor theileriosis vaccine for advance mass vaccination in the field on a large scale