Isolation and Characterization of Diazinon Degrading Bacteria from Contaminated Agriculture Soils

Background: Organic agricultural pesticides are so stable in ecosystems and biosphere. These compounds affect soil conditions and agricultural products. The aim of this study was isolation of diazinondegrading bacteria from Kerman pistachio orchards, Southeastern Iran. Methods: Diazinondegrading bacteria were enriched in BushnellHass medium. Identification and sequencing of prevalent degrading strains was performed using PCR based on lifying of 16s rDNA. Results: The soil of pistachio orchard has some degrading bacteria that are suitable for elimination of diazinon from soil and environment. Three diazinondegrading bacteria strains belong to: Pseudomonas putida strain D3, P. fluorescens strain D1 and Achromobacter piechaudii strain D8. The best degrading strain (D1), up to 100 ppm, illustrated a good growth, whereas more than this concentration, the growth was reduced. The results of GasChromatography (GC) confirmed the decomposition of organic pesticide by degradingbacteria. Conclusion: The results of GasChromatography (GC) confirmed the decomposition of organic pesticide by degradingbacteria. Using these strains and other biological reclamation methods we can eliminate bioenvironmental problems.