Other language title
Detection and differentiation of wild type and vaccine strains of canine distemper virus by a duplex reverse transcription polymerase chain reaction
Title of article
شناسايي و تفريق سويههاي وحشي و واكسن ويروس ديستمپر سگ سانان
Author/Authors
Dong، X. Y. نويسنده College of Veterinary Medicine,Department of Preventive Veterinary Medicine,South China Agricultural University,Guangzhou,China دونگ, زيااُ-يينگ , Li، W. H. نويسنده College of Veterinary Medicine,Department of Preventive Veterinary Medicine,South China Agricultural University,Guangzhou,China لي, وِن-هو , Zhu، J. L. نويسنده College of Veterinary Medicine,Department of Preventive Veterinary Medicine,South China Agricultural University,Guangzhou,China ژو, جون-لينگ , Liu، W. J. نويسنده College of Veterinary Medicine,Department of Preventive Veterinary Medicine,South China Agricultural University,Guangzhou,China ليو, وِن-جون , Zhao، M. Q. نويسنده College of Veterinary Medicine,Department of Preventive Veterinary Medicine,South China Agricultural University,Guangzhou,China ژااُ, مينگ-كيو , Luo، Y. W. نويسنده College of Veterinary Medicine,Department of Preventive Veterinary Medicine,South China Agricultural University,Guangzhou,China لواُ, يونگ-وِن , Chen، J. D. نويسنده College of Veterinary Medicine,Department of Preventive Veterinary Medicine,South China Agricultural University,Guangzhou,China چِن, جين-دينگ
Issue Information
فصلنامه با شماره پیاپی 51 سال 2015
Pages
4
From page
172
To page
175
Abstract
ويروس ديستمپر سگ سانان (CDV) عامل ديستمپر سگ سانان (CD) است كه بيماري شديد و بسيار واگيري در سگها ميباشد. در مطالعه حاضر، يك روش واكنش زنجيرهاي پليمراز داپلكس با رونويسي معكوس (RT PCR) براي شناسايي و تمايز سويههاي نوع وحشي و واكسن CDV تهيه شد. چهار پرايمر به منظور شناسايي و افتراق بين ويروسها به ترتيب به وسيله توليد فراوردههاي 638 و bp cDNA 781 طراحي شدند. علاوه بر اين، روش RT PCR دو رشتهاي براي شناسايي 67 نمونه مزرعه مشكوك به CD از استان گوانگ دونگ در چين استفاده گرديد. به عنوان نتيجه، 33 نمونه مشابه نوع وحشي بودند. روي هم رفته، روش RT PCR دو رشتهاي ويژگي و حساسيت بالايي دارد كه ميتواند براي شناسايي و تفريق مؤثر واكسن CDV و سويه نوع وحشي مورد استفاده قرار گيرد و نشان دهنده آن است كه ميتواند در شناسايي باليني و بررسي اپيدميولوژيكي به كار رود.
Abstract
Canine distemper virus (CDV) is the cause of canine distemper (CD) which is a severe and highly contagious disease in dogs. In the present study, a duplex reverse transcription polymerase chain reaction (RT PCR) method was developed for the detection and differentiation of wild type and vaccine strains of CDV. Four primers were designed to detect and discriminate the two viruses by generating 638 and 781 bp cDNA products, respectively. Furthermore, the duplex RT PCR method was used to detect 67 field sles suspected of CD from Guangdong province in China. Results showed that, 33 sles were to be wild type like. The duplex RT PCR method exhibited high specificity and sensitivity which could be used to effectively detect and differentiate wild type and vaccine CDV, indicating its use for clinical detection and epidemiological surveillance.
Keywords
Canine distemper virus , Differentiation , Duplex RT PCR , Specificity , Sensitivity
Journal title
Iranian Journal of Veterinary Research (IJVR)
Journal title
Iranian Journal of Veterinary Research (IJVR)
Record number
2398248
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